Journal article
Charge Has a Marked Influence on Hyperbranched Polymer Nanoparticle Association in Whole Human Blood
JJ Glass, L Chen, S Alcantara, EJ Crampin, KJ Thurecht, R De Rose, SJ Kent
ACS Macro Letters | AMER CHEMICAL SOC | Published : 2017
Abstract
In this study, we synthesize charge-varied hyperbranched polymers (HBPs) and demonstrate surface charge as a key parameter directing their association with specific human blood cell types. Using fresh human blood, we investigate the association of 5 nm HBPs with six white blood cell populations in their natural milieu by flow cytometry. While most cell types associate with cationic HBPs at 4 °C, at 37 °C phagocytic cells display similar (monocyte, dendritic cell) or greater (granulocyte) association with anionic HBPs compared to cationic HBPs. Neutral HBPs display remarkable stealth properties. Notably, these charge-association patterns are not solely defined by the plasma protein corona and..
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Grants
Awarded by Australian National Fabrication Facility
Funding Acknowledgements
This research was conducted and funded in large part by the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (CE140100036). The authors also acknowledge the Australian Research Council [FT110100284 (KJT), DP140100951 (KJT)], the National Health and Medical Research Council [APP1099321 (KJT), APP1041832 (SJK)], and the University of Queensland Collaboration and Industry Engagement Fund for funding. This work was performed in part at the Queensland node of the Australian National Fabrication Facility (ANFF), a company established under the National Collaborative Research Infrastructure Strategy. JJG acknowledges the support of an Australian Government Research Training Program Scholarship. LC would like to thank the Chinese Scholarship Council for financial support.